For consultation and training in image analysis, contact Federico Gasparoli. Federico is an expert microscopist and image analyst who provides free consultations on experimental design, pipeline development and data interpretation.

Jennifer Waters loves to teach.

• Jennifer’s YouTube channel, Microcourses
• Jennifer, Eva & Anna’s interactive remote microscopy course platform, Microtutor

Talley Lambert loves to code.

• FPbase.org – Fluorescent protein database and spectra viewer.
• Camera Simulation Engine – Camera simulator for learning camera principles
• iScopeCalc – Microscope calculator, calculate sampling rate, and spinning disc confocality.
• Spherical Aberration – Explore spherical aberration (ray diagram) as a function of refractive indices, NA, and sample depth

• Coverslips & coverslip-bottom dishes
• Fluorescence mounting media
• Choosing a fluorescent protein
• Critical controls when imaging multiple fluorophores
• Proper use of objective immersion oil

• What to put in the Materials & Methods section
• CITE Equipment Specifications
• Filter spectra and part numbers can generally be found in the FPbase spectra viewer embedded on the respective equipment page.