Michael

Widefield inverted fluorescence microscope with monochrome and color cameras.

Previous Configurations

Imaging modalities

  • Brightfield, including RGB color imaging

  • Widefield fluorescence

Hardware Components

  • Nikon Ti2 motorized inverted microscope with Perfect Focus System
  • Brightfield and epi-fluorescence optics

  • Hamamatsu Flash4.0 LT camera (6.5 µm2 photodiode)

  • Nikon motorized xy-stage

  • Lumencor Sola light engine for fluorescence illumination

  • NIS-Elements image acquisition software

  • Nikon DS-Fi3 color camera

Filter Spectra

Compare the fluorescence spectra of your fluorophore to the filters on this microscope.

Illumination Setting Excitation Emission
Widefield Blue 395/25 460/50
Widefield Green 466/40 525/50
Widefield Red 562/40 641/75
Widefield Far-Red 618/50 698/70