Lindsay

Total Internal Reflection Fluorescence (TIRF) on inverted microscope

Nikon Ti motorized inverted microscope with Perfect Focus System
Nikon TIRF illuminator
Prior Proscan III linear-encoded motorized stage, excitation and emission wheels and shutters
Agilent laser launch with : 405, 488, 561, & 647nm laser lines
NIS-Elements acquisition software
Hamamatsu ImagEM EM-CCD camera (16 µm² photodiode) with DualView for simultaneous 2-color
Hamamatsu ORCA-Quest qCMOS camera (4.6 µm² photodiode)
Tokai Hit 37°C, 5% CO2 stage-top incubation chamber
Lumencor SOLA light engine for fluorescence illumination

This instrument uses Class 3b lasers. All users must attend laser safety training.

Filters from Chroma

Compare the fluorescence spectra of your fluorophore to the filters on this microscope.