Gob

Widefield inverted fluorescence microscope with incubation chamber.

Imaging modalities

  • DIC
  • Phase contrast
  • Widefield fluorescence
  • Live cell imaging – long term

Hardware Components

  • Nikon Ti motorized inverted microscope with Perfect Focus System
  • DIC, phase, and epi-fluorescence optics

  • Hamamatsu ORCA-ER cooled CCD camera (6.45 µm2 photodiode)

  • Prior Proscan III motorized stage and shutters
  • Lumencor SOLA light engine for fluorescence illumination
  • Prior LED light source for transmitted light
  • MetaMorph image acquisition software
  • OkoLab 37°C, 5% CO2 cage microscope incubator

Filter Spectra

Filters from Chroma

Compare the fluorescence spectra of your fluorophore to the filters on this microscope.

Widefield Triple Cyan440/20475/20Widefield Triple FRET440/20540/21Widefield Triple Red575/22632/60

Optical Config Excitation Emission
CFP 436/20 480/40
GFP 480/40 535/50
YFP 500/20 535/30
RFP 560/40 630/75
Cy5 620/60 700/75
Widefield Triple Yellow 508/24 540/21