George Michael

Inverted fluorescence microscope optimized for fast acquisition and short-term live imaging, with a large variety of filter configurations

Previous Configurations

Imaging modalities

  • DIC
  • Phase contrast
  • Fluorescence
  • Ratio imaging: FRET, biosensors
  • Live cell imaging – short term
  • Image montages for large areas

Hardware Components

  • Nikon Ti2 motorized inverted microscope with Perfect Focus System

  • Hamamatsu ORCA-R2 cooled CCD camera (6.45 µm2 photodiode)

  • Nikon motorized XY-stage

  • Hamamatsu Flash4.0 V2+ sCMOS camera (6.5 µm2 photodiode)

  • Lumencor SpectraX fluorescence light source
  • MetaMorph image acquisition software
  • NIS-Elements Image Acquisition Software
  • Tokai Hit stage top incubator (37˚C and 5% CO2)

Filter Spectra

Compare the fluorescence spectra of your fluorophore to the filters on this microscope.

Optical Config Excitation Dichroic Emission
Widefield Blue 395/25 T425lpxr ET460/50m
Widefield Dual Green 470/24 59022bs BrightLine 525/50
Widefield Dual Red 575/25 59022bs BrightLine 641/75
Widefield Dual FRET 470/24 59022bs BrightLine 641/75
Widefield Triple Cyan 440/20 69008bs ET470/24m
Widefield Triple Yellow ET500/20x 69008bs ET535/30mm
Widefield Triple Red 575/25 69008bs BrightLine 641/75
Widefield Triple FRET 440/20 69008bs ET535/30mm
Widefield Triple Red 640/30 T660lpxr BrightLine 698/70