George

Yokogawa spinning disk confocal on an inverted Nikon Ti fluorescence microscope with incubation enclosure.

Previous Configurations

Imaging modalities

  • DIC
  • Phase contrast
  • Widefield fluorescence
  • Spinning disk confocal
  • Live cell imaging

Hardware Components

  • Nikon Ti motorized inverted microscope with Perfect Focus System

  • Yokagawa CSU-X1 spinning disk confocal

  • Nikon LUN-F XL solid state laser combiner: 405 (80mW), 445 (35mW), 488 (80mW), 514 (50mW), 561 (65mW), 640nm (60mW)

  • Hamamatsu ORCA-Fusion BT sCMOS camera (6.5 µm2 photodiode)

  • Prior Proscan II motorized stage and shutters

  • NIS Elements image acquisition software

  • Lumencor SOLA fluorescence light source
  • OkoLab 37°C, 5% CO2 cage microscope incubator

Filter Spectra

Filters from Chroma

Compare the fluorescence spectra of your fluorophore to the filters on this microscope.

Illumination Setting Excitation Dichroic Emission
447 447nm 447/515/642 Triple 480/40
491 491nm 405/491/561/642 Quad 525/50
561 561nm 405/491/561/642 Quad 620/60
561 TMR 561nm 405/491/561/642 Quad 605/70
642 642nm 405/491/561/642 Quad 700/75
Widefield FITC 480/40 505lp 535/50
Widefield TRITC 540/25 595lp 620/60
Widefield CFP 436/20  455lp 480/40
Widefield YFP 500/20  515lp 535/30