Gene ParmesanTalley2024-12-13T09:57:38-05:00 Gene ParmesanWidefield inverted fluorescence microscope with incubation chamber. Imaging modalitiesDICPhase contrastWidefield fluorescenceLive cell imaging – long termHardware ComponentsNikon Ti2 motorized inverted microscope with Perfect Focus SystemDIC, phase, and epi-fluorescence optics Hamamatsu Flash4.0 sCMOS camera (6.5 µm2 photodiode) Nikon motorized stage and emission filter wheel Lumencor Sola light engine for fluorescence illumination NIS Elements image acquisition software Tokai Hit 37° C, 5% CO2 cage microscope incubator Filter SpectraFilters from Chroma