Warning: This is an archival page that does not reflect the current equipment on this microscope. To see the current microscope configuration, click here.

Buster

Widefield inverted Nikon Ti fluorescence microscope with incubation chamber.

Imaging modalities

DIC
Phase contrast
Widefield fluorescence
Live cell imaging – long term

Hardware Components

Nikon Ti motorized inverted microscope with Perfect Focus System
DIC, phase, and epi-fluorescence optics
Hamamatsu ORCA-R2 cooled CCD camera
Nikon motorized stage and filter wheels
Lumencor SOLA light engine for fluorescence illumination
NIS Elements image acquisition software
OkoLab 37° C, 5% CO2 cage microscope incubator

Filter Spectra

Filters from Chroma

Interactive Spectra
Table

Interactive Spectra

Compare the fluorescence spectra of your fluorophore to the filters on this microscope.

Table

Optical Config	Excitation	Emission
DAPI	395/25	460/50
GFP	480/40	535/50
RFP-mCherry	560/40	630/75
Quad-DAPI	402/15	455/50
Quad-FITC	490/20	525/36
Quad-TRITC	555/25	605/52
Quad-Cy5	645/30	705/72
Quad 402-525	402/15	525/36
Dual-CFP	430/24	470/24
Dual-YFP	500/20	535/30
Dual-FRET	430/24	535/30