Buster

Widefield inverted fluorescence microscope with incubation chamber.

Previous Configurations

Imaging modalities

  • DIC
  • Phase contrast
  • Widefield fluorescence
  • Live cell imaging – long term

Hardware Components

  • Nikon Ti2 motorized inverted microscope with Perfect Focus System
  • DIC, phase, and epi-fluorescence optics

  • Hamamatsu Flash4.0 sCMOS camera (6.5 µm2 photodiode)

  • Nikon motorized stage and emission filter wheel
  • Lumencor Spectra-X light engine for fluorescence illumination
  • NIS Elements image acquisition software
  • OkoLab 37° C, 5% CO2 cage microscope incubator

Filter Spectra

Filters from Chroma

Compare the fluorescence spectra of your fluorophore to the filters on this microscope.

Optical Config Excitation Emission
DAPI 395/25 460/50
GFP 480/40 535/50
RFP-mCherry 560/40 630/75
Quad-DAPI 402/15 455/50
Quad-FITC 490/20 525/36
Quad-TRITC 555/25 605/52
Quad-Cy5 645/30 705/72
Quad 402-525 402/15 525/36
Dual-CFP 430/24 470/24
Dual-YFP 500/20 535/30
Dual-FRET 430/24 535/30