Gene Parmesan

Widefield inverted fluorescence microscope with incubation chamber.

Imaging modalities

  • DIC
  • Phase contrast
  • Widefield fluorescence
  • Live cell imaging – long term

Hardware Components

  • Nikon Ti2 motorized inverted microscope with Perfect Focus System
  • DIC, phase, and epi-fluorescence optics

  • Hamamatsu Flash4.0 sCMOS camera (6.5 µm2 photodiode)

  • Nikon motorized stage and emission filter wheel

  • Lumencor Sola light engine for fluorescence illumination

  • NIS Elements image acquisition software

  • Tokai Hit 37° C, 5% CO2 cage microscope incubator

Filter Spectra

Filters from Chroma

Compare the fluorescence spectra of your fluorophore to the filters on this microscope.

Illumination Setting Excitation Dichroic Emission
442 442nm 447/515/642 Triple 480/40
488 488nm 405/491/561/642 Quad 525/50
515 515nm 447/515/642 Triple 540/30
561 561nm 405/491/561/642 Quad 620/60
642 642nm 405/491/561/642 Quad 700/75
Widefield DAPI 395/25 400lp 460/25
Widefield FITC 480/40 505lp 535/50
Widefield TRITC 560/40 595lp 630/60