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George Michael

Inverted Nikon Ti fluorescence microscope optimized for fast acquisition and slide scanning with a large variety of filter configurations

Imaging modalities

DIC
Phase contrast
Fluorescence
Ratio imaging: Fura, FRET, biosensors
Live cell imaging – short term
Image montages for large areas

Hardware Components

Nikon Ti motorized inverted microscope with Perfect Focus System
Hamamatsu ORCA-R2 cooled CCD camera
Prior Proscan III linear-encoded motorized stage, excitation and emission wheels and shutters
Prior LumenPro fluorescence light source with excitation and attenuation wheels
MetaMorph image acquisition software
Tokai Hit stage top incubator (37˚C and 5% CO2)

Filter Spectra

Filters from Chroma

Table

Table

Optical Config	Excitation	Dichroic	Emission
Filter DAPI	AT 350/50	400lp	ET 460/50
Filter FITC	HQ 480/40	505lp	ET 535/50
Filter TRITC	ET 545/30	570lp	ET 620/60
Filter Cy5	ET 620/60	660lp	ET 700/75
Wheel UV	ET 402/15	69002bs	ET 460/40
Wheel CFP	ET 430/24	69008bs	ET 470/24
Wheel GFP	ET 490/20	69002bs	ET 535/50
Wheel YFP	ET 500/20	69008bs	ET 535/30
Wheel FRET	ET 430/24	69008bs	ET 535/30
Wheel RFP	ET 572/35	69002bs	ET 632/60
Fura 340	ET 340x	400lp	ET 510/80
Fura 380	ET 380x	400lp	ET 510/80