Barry Zuckerkorn (Retired)

Barry Zuckerkorn (Retired)Talley2024-09-12T14:32:24-04:00

Barry Zuckerkorn (Retired 9/2024)

Widefield inverted fluorescence microscope with monochrome and color cameras.

Imaging modalities

Brightfield, including RGB color imaging
Widefield fluorescence

Hardware Components

Nikon Ti2 motorized inverted microscope with Perfect Focus System
Brightfield and epi-fluorescence optics
Hamamatsu Flash4.0 LT camera (6.5 µm² photodiode)
Nikon DS-Fi3 color camera
Lumencor Sola light engine for fluorescence illumination
Nikon white light LED for brightfield illumination
Nikon motorized xy-stage
NIS-Elements image acquisition software

Filter Spectra

Interactive Spectra
Table

Interactive Spectra

Compare the fluorescence spectra of your fluorophore to the filters on this microscope.

Table

Illumination Setting	Excitation	Emission
Widefield Blue	395/25	460/50
Widefield Green	466/40	525/50
Widefield Red	562/40	641/75
Widefield Far-Red	618/50	698/70