Michael (Before 11/25/2017)

Michael (Before 11/25/2017)Talley2018-12-21T12:43:27-05:00

Warning: This is an archival page that does not reflect the current equipment on this microscope. To see the current microscope configuration, click here.

Michael

Widefield inverted Nikon Ti fluorescence microscope with incubation chamber.

Imaging modalities

DIC
Phase contrast
Widefield fluorescence
Live cell imaging – long term

Hardware Components

Nikon Ti motorized inverted microscope with Perfect Focus System
DIC, phase, and epi-fluorescence optics
Hamamatsu ORCA-ER cooled CCD camera
Prior Proscan III motorized stage and shutters
Lumencor Spectra-X light engine for fluorescence illumination
MetaMorph image acquisition software
OkoLab 37° C, 5% CO2 cage microscope incubator

Filter Spectra

Filters from Chroma

Table

Table

Illumination Setting	Excitation	Dichroic	Emission
Camera_405x515m	395/25	Lumencor Quad	515/30
Camera_GFP	470/30	Lumencor Quad	515/30
Camera_GFPexRFPem	470/30	Lumencor Quad	632/60
Camera_RFP	575/22	Lumencor Triple	632/60
Camera_RFPexCy5em	575/22	Lumencor Quad	705/72
Camera_Cy5	640/30	Lumencor Quad	705/72
Camera_CFP	440/20	Lumencor Triple	475/20
Camera_YFP	508/24	Lumencor Triple	540/21
Camera_FRET	440/20	Lumencor Triple	540/21
Eye_GFP	470/30	B-2E/C	535/40
Eye_RFP	575/22	HcRED1	640/50
Eye_YFP	508/24	HQ:Y GFP	535/30