For help with image analysis, contact the Image Analysis Collaboratory

Jennifer Waters loves to teach.

• Jennifer’s YouTube channel, Microcourses
• Jennifer, Eva & Anna’s interactive remote microscopy course platform, Microtutor

Talley Lambert loves to code.

• FPbase.org – Fluorescent protein database and spectra viewer.
• Camera Simulation Engine – Camera simulator for learning camera principles
• iScopeCalc – Microscope calculator, calculate sampling rate, and spinning disc confocality.
• Spherical Aberration – Explore spherical aberration (ray diagram) as a function of refractive indices, NA, and sample depth

• Coverslips & coverslip-bottom dishes
• Fluorescence mounting media
• Choosing a fluorescent protein
• Critical controls when imaging multiple fluorophores
• Proper use of objective immersion oil

• What to put in the Materials & Methods section
• CITE Equipment Specifications
• Filter spectra and part numbers can generally be found in the FPbase spectra viewer embedded on the respective equipment page.