Tobias

Nikon 3D N-STORM Super-resolution / TIRF microscope

This microscope retired in April 2024, and is no longer available for use.

Imaging modalities

  • DIC
  • Phase contrast
  • TIRF
  • STORM super-resolution imaging
  • 3D-STORM super-resolution imaging

Hardware Components

  • Nikon Ti motorized inverted microscope with Perfect Focus System

  • Nikon TIRF illuminator
  • Nikon 3D-STORM system
  • Agilent MLC400B laser launch with 405nm (20mW), 488nm (50mW), 561nm (50mW) & 647nm (125mW) power (measured at fiber optic)

  • Andor DU-897 EM-CCD camera (16 µm2 photodiode)

  • Hamamatsu ImagEM (16 µm2 photodiode) with dualView

  • Prior Proscan II filter wheels and shutters

  • Lumencor SOLA fluorescence light source

Filter Spectra

Filters from Chroma

Compare the fluorescence spectra of your fluorophore to the filters on this microscope.

Tobias has 4 laser lines: 405, 488, 561, and 647nm

Optical Config Excitation Dichroic Notch Emission
TIRF 488 ZET405/488/561/647x ZT405/488/561/647rpc ZET405/488/561/647m ET 525/50 M
TIRF 561 ZET405/488/561/647x ZT405/488/561/647rpc ZET405/488/561/647m ET 600/50 M
TIRF 647 ZET405/488/561/647x ZT405/488/561/647rpc ZET405/488/561/647m ET 700/75 M